Strain Data Sheet

RBRC02140

Strain Information

Image
BRC No.RBRC02140
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(DBH-tauGFP)17
Former Common nameDBH-tauGFP17
H-2 Haplotype
ES Cell line
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were backcrossed to C57BL/6J over 20 times. Line 17 (RBRC02140), line 36 (RBRC02141).
Strain descriptionThese transgenic mice express a fusion gene of bovine tau protein and GFP under the control of the human DBH promoter in typical noradrenergic and adrenergic cell groups. Line 17 and 36 were generated. The expression level of Tg in each line was following order: 17 > 36 (developer’s information).
Colony maintenance
ReferencesNeurogastroenterol. Motil., 22(3):331-e86 (2010). 19775251

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNhuman dopamine beta hydroxylase (DBH) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
taubovine tau protein cDNAUNtauhuman dopamine beta hydroxylase (DBH) promoter

Ordering Information

Donor DNAhuman dopamine beta-hydroxylase(DBH) promoter, rabbit beta-globin intron, bovine tau protein cDNA, jellyfish green fluorescent protein (GFP) cDNA, rabbit beta- globin poly A, SV40 early gene poly A
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neurogastroenterol. Motil. 22(3):331-e86. PMID: 19775251
DepositorKazuto Kobayashi (Fukushima Medical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data