Strain Data Sheet
Data update: Jul 19, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC02176  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129S2-Clgn<tm1Osb>  
Former Common name B6-Clgn KO  
H-2 Haplotype No Data  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Masato Ikawa, Research Institute for Microbial Diseases, Osaka University in 1995. D3 ES cells derived from 129S2/SvPas were used. The mutant mice were backcrossed to C57BL/6.  
Strain description Clgn, calmegin gene knockout mice. A neomycin selection cassette was inserted into the exon 2 of the Clgn gene. Homozygous mutant males were infertile.  
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrSlc]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Clgn  
Symbol name calmegin  
Chromosome 8  
Common name 4930459O04Rik, Cln  
Symbol description No Data  
 2 Symbol poly A  
Symbol name mouse PGK poly A  
Chromosome 8  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 8  
Common name neo; neomycin;  
Symbol description No Data  
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 387, 607-611 (1997).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University ( The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Okabe, Masaru (Osaka university)  Okabe, Masaru 
Strain Status /
(Expected delivery)

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