Strain Information | |
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Image | |
BRC No. | RBRC02189 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6Cr-Tg(Pcdh21-cre)BYoko |
Former Common name | Pcdh21-nCre, C57BL/6 |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrSlc |
Appearance | black [a/a B/B C/C] |
Strain development | Developed by Dr. Mineto Yokoi Kyoto University Guraduate School of Medicine nin 2004. |
Strain description | This transgenic mice express the cre recombinase gene under the control of the Pcdh21 gene promoter. This can be used to make an conditional knock-out by crossing with the other genetically-engineered mice that have floxed genes. The expression of cre recombinase was observed in the mitral/tufted cells that are projection neurons in the olfactory bulb. |
Colony maintenance | Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc) |
References | Genesis, 43, 12-16 (2005). 16106355 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcrePcdh21 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)UNtTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domainPcdh21 promoter |
Ordering Information | |
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Donor DNA | FLAG tag, tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain), human FGF2-IRES, nuclear localization signal, Phage P1 Cre recombinase, rabbit beta-globin intron/polyA signal, mouse pcdh21 genomic DNA |
Research application | Cre/loxP system Neurobiology Research Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 43, 12-16 (2005). |
Depositor | Yokoi Mineto (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Fujimoto S, Leiwe MN, Aihara S, Sakaguchi R, Muroyama Y, Kobayakawa R, Kobayakawa K, Saito T, Imai T. Activity-dependent local protection and lateral inhibition control synaptic competition in developing mitral cells in mice. Dev Cell (2023) 37290446 Inoue N, Nishizumi H, Ooyama R, Mogi K, Nishimori K, Kikusui T, Sakano H. The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli. Elife 10 (2021) 33780330 Miller JE, Granados-Fuentes D, Wang T, Marpegan L, Holy TE, Herzog ED. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34(17) 6040-6(2014) 24760863 Iwata R, Kiyonari H, Imai T. Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb. Neuron 96(5) 1139-1152.e7(2017) 29216451 |