Strain Information | |
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Image | |
BRC No. | RBRC02228 |
Type | Transgene |
Species | Mus musculus |
Strain name | ICR.Cg-Tg(TOPGAL)/YsaRbrc |
Former Common name | TOP-GAL |
H-2 Haplotype | |
ES Cell line | |
Background strain | ICR |
Appearance | albino [c/c] |
Strain development | Developed by Yumiko Saga, National Institute of Genetics in 2005. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F2 fertilized eggs. ICR mixed background. |
Strain description | Transgenic mice for visualizing Wnt signal pathway. Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt beta-catenin or so-called Wnt canonical signaling pathways. The TOP-GAL transgenic mice are a reporter line which can visualize the Wnt canonical pathway by beta-galactosidase activity. The transgenic mice were generated with ins-TOPGAL that accurately drive beta-galactosidase under TCF/beta-catenin. These transgenic mice will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo. |
Colony maintenance | Carrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]. Tg mice are viable and fertile. |
References | Genesis, 45, 90-100 (2007) 17245775 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)UNTOPFLUSH promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnls |
Ordering Information | |
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Donor DNA | TCF binding site from TOPFLUSH vector (Upstate) , HSV TK promoter, E. coli LacZ, SV40-late-poly A, chicken β-globin HS4 insulater, Simian virus 40 Large T antigen nuclear localization signal (nls) |
Research application | Developmental Biology Research Fluorescent Proteins/lacZ System General Purpose |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 45, 90-100 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yumiko Saga (National Institute of Genetics) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Aug 2009 Genetic Background Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R. Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after γ-irradiation through Wnt/β-catenin pathway. Carcinogenesis 36(6) 622-31(2015) 25827435 Garbe DS, Ring RH. Investigating tonic Wnt signaling throughout the adult CNS and in the hippocampal neurogenic niche of BatGal and ins-TopGal mice. Cell Mol Neurobiol 32(7) 1159-74(2012) 22491991 Lu TL, Chen CM. Differential requirements for β-catenin in murine prostate cancer originating from basal versus luminal cells. J Pathol 236(3) 290-301(2015) 25712462 Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y. β-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. Sci Rep 4 6959(2014) 25376241 |