Strain Information | |
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Image | |
BRC No. | RBRC02295 |
Type | Targeted Mutation and Transgene |
Species | Mus musculus |
Strain name | B6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy |
Former Common name | Cas-ex2<flox/flox>-Tg(BLG-Cre) |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003. |
Strain description | Casp130 (Bcar1) gene exon 2 floxed and BLG (beta lactoglobulin)-cre double mutant mice. Viable and fertile, but the reproductive performance is not so good. |
Colony maintenance | KO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj] |
References | Hepatology, 52(3), 1089-1099 (2010). 20623582 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Bcar1breast cancer anti-estrogen resistance 18Bcar1<tm1.2Homy>targeted mutation 1.2, HomyCas, Crkas, p130 Cas Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site8Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP8loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcresheep BLG promoter |
Ordering Information | |
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Donor DNA | phage P1 loxP sites, yeast (Saccharomyces cerevisiae) FRT site, mouse Cas genomic DNA, sheep BLG genomic DNA, phage P1 Cre-recombinase |
Research application | Cell Biology Research Cre/loxP system FLP/frt system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
Depositor | Hiroaki Honda (Tokyo Women's Medical University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |