Strain Information | |
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Image | |
BRC No. | RBRC02348 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Fabp7<tm1(crePR)Ksak> |
Former Common name | BFABP-CrePR |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | Developed by Mika Tsujita, Rie Natsume and Kenji Sakimura, Brain Research Institute, Niigata University in 2004. RENKA ES cells derived from C57BL/6NCrj were used. C57BL/6N background. |
Strain description | Fabp7 CrePR knock-in mice. A CrePR sequence was integrated into the start methionine site of the B-FABP gene at an in-frame position. A non-coding and pgk-neo cassette sequence are located downstream from CrePR. The pgk-neo cassette can be removed, as it has an frt sequence on both sides. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fabp7fatty acid binding protein 7, brain10Fabp7<tm1(crePR)Ksak>targeted mutation 1, Kenji SakimuraB-FABP, Blbp Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site10Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PGRProgesterone receptor (human)10PGR Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase10cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Amouse pgk polyA10poly A |
Ordering Information | |
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Donor DNA | Simian virus 40 Large T antigen nuclear localization signal (NLS), phage P1 Cre, human progesterone receptor cDNA, human G-CSF polyadenylation signal sequence (pA), mouse phosphoglycerokinase (Pgk1) promoter, E. coli neo, mouse Pgk1 poly A, S. cerevisiae 2 micro plasmid FRT sites |
Research application | Cre/loxP system FLP/frt system General Purpose Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-auther in articles when the users publish their data using these mice for 2 year after provided to the RIKEN BRC. Users should contact the DEPOSITOR in the case of application for any patents with the results from these mice. |
Depositor | Kenji Sakimura (Niigata University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |