Strain Data Sheet

RBRC02396

Strain Information

Image
BRC No.RBRC02396
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129X1-Pknox1<tm1.1Nuk>
Former Common namePrep1LacZKIdeltaNeo, C57BL/6
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Atsushi Kuroiwa, Nagoya University.
Strain descriptionPrep1 (Pknox1) knockout mice. Prep1 gene is a member of the TALE class of homeodomain proteins and essential in embryonic development. Homozygous mutant mice are embryonic lethal (E9.5). This strain is useful for elucidation of the function of Prep1 gene during embryonic development.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pknox1Pbx/knotted 1 homeobox17Pknox1<tm1.1Nuk>targeted mutation 1.1, Nagoya University Atsushi KuroiwaD17Wsu76e, PREP1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly ASV40 polyA17poly A

Ordering Information

Donor DNAphage P1 loxP, E. coli LacZ, SV40 polyA signal sequence, mouse Pknox1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorAtsushi Kuroiwa (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data