Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
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RBRC No. RBRC02396  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name B6.129X1-Pknox1<tm1.1Nuk>  
Former Common name Prep1LacZKIdeltaNeo, C57BL/6  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Dr. Atsushi Kuroiwa, Nagoya University.  
Strain description Prep1 (Pknox1) knockout mice. Prep1 gene is a member of the TALE class of homeodomain proteins and essential in embryonic development. Homozygous mutant mice are embryonic lethal (E9.5). This strain is useful for elucidation of the function of Prep1 gene during embryonic development.  
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Pknox1  
Symbol name Pbx/knotted 1 homeobox  
Chromosome 17  
Common name D17Wsu76e, PREP1  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 17  
Common name No Data  
Symbol description E. coli beta galactosidase  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 4 Symbol poly A  
Symbol name SV40 poly A  
Chromosome 17  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Kuroiwa, Atsushi (Graduate School of Science, Nagoya University)  Kuroiwa, Atsushi 
Strain Status /
Availability
(Expected delivery)

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