Strain Data Sheet

RBRC02402

Strain Information

Image
BRC No.RBRC02402
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Bst1<tm2Thir> Cd38<tm1Thir>
Former Common nameBST-1/CD157 CD38 double KO
H-2 Haplotype
ES Cell lineEB5 [129/Ola]
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Katsuhiko Ishihara and Toshio Hirano, Osaka University. Backcrossed to C57BL/6N for 4 times, and then to C57BL/6J.
Strain descriptionCD157 (Bst1) and CD38 double knockout mice.
Colony maintenanceDouble heterozygote x Wild-type [C57BL/6JJcl]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bst1bone marrow stromal cell antigen 15Bst1<tm2Thir>targeted mutation 2, Toshio Hirano114/A10, Bp3, Bsta1, CD157, Ly65

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cd38CD38 antigen5Cd38<tm1Thir>targeted mutation 1, Toshio HiranoCd38-rs1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5neo; neomycin;herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)5puro

Ordering Information

Donor DNAStreptomyces alboniger puromycin resistance gene, mouse Bst1 genomic DNA, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Cd38 genomic DNA
Research application
Specific Term and Conditionsa) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR, Dr. Katsuhiko ISHIHARA (ishihara-im@med.kawasaki-m.ac.jp). b) RECIPIENT who wants to use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Kawasaki medical school.
DepositorKatsuhiko Ishihara (Kawasaki Medical School)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data