Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
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RBRC No. RBRC02405  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.Cg-4930583H14Rik<tm1Hmd>  
Former Common name 4930583H14Rik flox  
H-2 Haplotype No Data  
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.  
Strain description 4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome 3  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 2 Symbol 4930583H14Rik  
Symbol name RIKEN cDNA 4930583H14 gene  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 4 Symbol Myc-tag  
Symbol name myc-epitope tag  
Chromosome 3  
Common name No Data  
Symbol description No Data  
 5 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 3  
Common name No Data  
Symbol description E. coli beta galactosidase  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 3  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 7 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 3  
Common name neo; neomycin;  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology)  Hamada, Hiroshi 
Strain Status /
Availability
(Expected delivery)

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