Strain Data Sheet
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC02412  
Type Transgene CongenicCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Tg(Nes-cre)1Nogu  
Former Common name B6.Cg-Tg(Nes-cre)1Nogu  
H-2 Haplotype No Data  
Background strain C57BL/6JJcl  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Shigeru Noguchi, Meiji Dairies Corporation and Kanagawa Academy of Science and Technology and Dr. Toshikuni Sasaoka, Center for Bioresource-based Researches, Brain Research Institute, Niigata University. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6.  
Strain description Nestin-cre transgenic mice, expressing a cre recombinase gene under the control of Nestin promoter and enhancer. This Nestin-cre transgene contains a rat nestin promoter, cre recombinase gene, IRES-lacZ-polyA and neuron specific enhancer (around 100 bp of exon 1, the entire intron 1 and around 100 bp of exon 3 of nesitin gene). Tissue-specific expression: observed in whole brain, spinal cord and testis/ovary; not observed in thymus, lung, heart, kidney, spleen, stomach, small and large intestine, pancreas, skeletal muscle and tail (Developers, personal communication).  
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Nes  
Symbol name nestin  
Chromosome UN  
Common name ESTM46  
Symbol description No Data  
 2 Symbol IRES  
Symbol name internal ribosomal entry site (EMCV)  
Chromosome UN  
Common name internal ribosomal entry site from EMCV  
Symbol description No Data  
 3 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome UN  
Common name No Data  
Symbol description No Data  
 4 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome UN  
Common name No Data  
Symbol description E. coli beta galactosidase  
 5 Symbol poly A  
Symbol name SV40 poly A signal  
Chromosome UN  
Common name No Data  
Symbol description No Data  
References No Data  
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of academic research. Prior to publication, the RECIPIENT should contact the developer scientist, Dr. Shigeru Noguchi and acknowledge Drs. Shigeru Noguchi and Dr. Toshikuni Sasaoka.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor Nishimura, Koichiro (Kanagawa Institute of Industrial Science and Technology)  Nishimura, Koichiro 
Strain Status /
(Expected delivery)

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