Strain Data Sheet

RBRC02529

Strain Information

Image
BRC No.RBRC02529
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Pcdha<tm(dupSR2)>/TyagRbrc
Former Common nameCNR SR2dupli delta neo, SR2dupli deltaNeo, SR2dupli delta neo
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. TT2 ES cells were used to generate the mutant mice. Frt flanked neo cassette was removed by crossing with FLP mice.
Strain descriptionThe region od SR2 of the CNR (Pcdha) gene was duplicated.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcdhaprotocadherin alpha cluster18Pcdha<tm(dupSR2)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNAFRT、loxP
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data