Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Aug 20, 2019
Health Report   PCR Protocol   Thawing Method
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  Mouse Marking
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RBRC No. RBRC02565  
Type Targeted MutationCartagena   wks
Species Mus musculus  
Strain name ICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc  
Former Common name Nanos3-CrepA(neo)#35  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Dr. Yumiko Saga (2007).  
Strain description These knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile.  
Colony maintenance Sibling Mating (Hemizygote x Wild-type)  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Nanos3  
Symbol name nanos homolog 3 (Drosophila)  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 4 Symbol cre  
Symbol name Phage P1 Cre recombinase  
Chromosome 8  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 8  
Common name neo; neomycin;  
Symbol description No Data  
References Dev Biol. 2008 Jun 1;318(1):133-42. Epub 2008 Mar 20.  
Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 318, 133-142 (2008).
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER's names as co-authors.  
Additional information
 1 Saga Lab HP  
 2 Genotyping protocol <PCR>  
Depositor Saga, Yumiko (National Institute of Genetics)  Saga, Yumiko 
Strain Status /
Availability
(Expected delivery)

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