Strain Data Sheet

RBRC02653

Strain Information

Image
BRC No.RBRC02653
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Tsix/Xist<tm3Sado>
Former Common nameTsix<pA>; B6.Cg-Tsix/Xist<tm3Sado>
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Takashi Sado, National Institute of Genetics in 2006. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain descriptionTsix<pA> mice. The fragment containing a second intron of the human gamma-globin gene, which harbors an mpA cassette in an antisense orientation with respect to Xist transcription was inserted into exon 1 of Xist. Antisense transcription across the Xist promoter is diminished on this allele. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenanceHeterozygote (female) x Wild-type (male) [C57BL/6JJcl]
ReferencesDevelopment 135, 227-235 (2008). 18057104

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TsixX (inactive)-specific transcript, antisenseXTsix<tm3Sado>targeted mutation 3, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xistinactive X specific transcriptsXXist<tm3Sado>targeted mutation 3, Takashi Sado

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SASplice acceptor (human)XSA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Splice donorsplicing donar (human)XSplice donor

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
poly Amultiple polyA signals (SV40)Xpoly A

Ordering Information

Donor DNAmouse Xist genomic DNA, Phage P1 loxP, poly A (multiple poly A signals) SV40, splicing acceptor (human), splicing donar (human)
Research applicationCre/loxP system
Developmental Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
DepositorTakashi Sado (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Additional Info.Mouse of the Month Feb 2009
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)

BRC mice in Publications

No Data