Strain Information | |
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Image | |
BRC No. | RBRC02653 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;129-Tsix/Xist<tm3Sado> |
Former Common name | Tsix<pA>; B6.Cg-Tsix/Xist<tm3Sado> |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Takashi Sado, National Institute of Genetics in 2006. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6). |
Strain description | Tsix<pA> mice. The fragment containing a second intron of the human gamma-globin gene, which harbors an mpA cassette in an antisense orientation with respect to Xist transcription was inserted into exon 1 of Xist. Antisense transcription across the Xist promoter is diminished on this allele. Heterozygote mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655). |
Colony maintenance | Heterozygote (female) x Wild-type (male) [C57BL/6JJcl] |
References | Development 135, 227-235 (2008). 18057104 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SASplice acceptor (human)XSA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Splice donorsplicing donar (human)XSplice donor Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter poly Amultiple polyA signals (SV40)Xpoly A |
Ordering Information | |
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Donor DNA | mouse Xist genomic DNA, Phage P1 loxP, poly A (multiple poly A signals) SV40, splicing acceptor (human), splicing donar (human) |
Research application | Cre/loxP system Developmental Biology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. |
Depositor | Takashi Sado (National Institute of Genetics) |
Strain Status | Frozen embryos |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) |
Additional Info. | Mouse of the Month Feb 2009 Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |