Strain Data Sheet
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Data update: Apr 19, 2019
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RBRC No. RBRC02654  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6;129-Xist<tm4Sado>  
Former Common name Xist; B6.Cg-Xist  
H-2 Haplotype No Data  
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Takashi Sado, National Institute of Genetics in 2006. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).  
Strain description Xist<IVS19> mice. A second intron of the human gamma-globin gene was inserted into exon 1 of Xist gene. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).  
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Xist  
Symbol name inactive X specific transcripts  
Chromosome X  
Common name No Data  
Symbol description No Data  
 2 Symbol SA  
Symbol name Splice acceptor (human)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 3 Symbol Splice donor  
Symbol name splicing donar (human)  
Chromosome X  
Common name No Data  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Development. 2008 Jan;135(2):227-35. Epub 2007 Dec 5.  
Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification.
Research applications Cre/loxP system,
Developmental Biology Research  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Feb 2009  
 3 Genotyping protocol <PCR>  
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)  
Strain Status /
Availability
(Expected delivery)

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