Strain Information | |
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Image | |
BRC No. | RBRC02657 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito> |
Former Common name | ROSA nls LacZ |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J. |
Strain description | PGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | Front. Behav. Neurosci., 7:17 (2013). 23487260 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/09Room:3-ARack:K 2023/10/10Room:3-ARack:K 2023/07/10Room:3-ARack:K 2023/04/10Room:3-ARack:K 2023/01/10Room:3-ARack:K 2022/10/11Room:3-ARack:K 2022/07/11Room:3-ARack:K 2022/04/11Room:3-ARack:K |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>targeted mutation 1, Shigeyoshi Itohara Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 nuclear localaization signal (chemically-synthesized nucleotide)6nls |
Ordering Information | |
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Donor DNA | synthetic splice acceptor sequence, P1 phage loxP sites, STOP (mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, polyA), Simian virus 40 Large T antigen nuclear localization signal (NLS), E.coli lacZ, SV40 polyA signal, mouse ROSA26 genomic DNA |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17, (2013). |
Depositor | Shigeyoshi Itohara (RIKEN) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Mouse of the Month Mar 2013 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Namiki T, Kamoshita M, Kageyama A, Terakawa J, Ito J, Kashiwazaki N. Utility of progesterone receptor-ires-Cre to generate conditional knockout mice for uterine study. Anim Sci J 92(1) e13615(2021) 34402137 Yoshimura Y, Ida-Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, Yagoto M, Kawai K, Takahashi T, Nakayama M, Ito M. Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res 27(2) 193-201(2018) 29546522 |