Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC02657  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>  
Former Common name ROSA nls LacZ  
H-2 Haplotype No Data  
ES cell line E14 [129P2/OlaHsd]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J.  
Strain description PGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Gt(ROSA)26Sor  
Symbol name gene trap ROSA 26, Philippe Soriano  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 6  
Common name No Data  
Symbol description E. coli beta galactosidase  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 4 Symbol nls  
Symbol name SV40 nuclear localaization signal (chemically-synthesized nucleotide)  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 5 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References   
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Front. Behav. Neurosci., 7:17, (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.  
Additional information
 1 Lab HP  
 2 Mouse of the Month Mar 2013  
 3 Genotyping protocol <PCR>  
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science)  Itohara, ShigeYoshi 
Strain Status /
Availability
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