Strain Data Sheet

RBRC02658

Strain Information

Image
BRC No.RBRC02658
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gt(ROSA)26Sor<tm1(GAP-lacZ)Ito>
Former Common nameROSA GAP LacZ
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used to generate the mutant mice. The mice were crossed to C57BL/6J (C57BL/6 semicongenic).
Strain descriptionPGK-neo cassette flanked by loxP sites and GAP-lacZ were inserted into the Gt(ROSA)26Sor locus. The GAP-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized along axonal projections.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesFront. Behav. Neurosci., 7:17 (2013). 23487260

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(GAP-lacZ)Ito>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mouse synthetic GAP signal; synthetic splice acceptor sequence6

Ordering Information

Donor DNAROSA26, PGK promoter, mouse synthetic GAP signal; synthetic splice acceptor sequence; loxP-P1 phage; poly A signal - SV40; lacZ, Neo -E.coli (PGKneo flanked by loxP, and GAP-LacZ insertion in the ROSA26 locus.)
Research applicationCell Biology Research
Cre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Behav. Neurosci., 7:17 (2013).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data