Strain Data Sheet

RBRC02682

Strain Information

Image
BRC No.RBRC02682
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Trsp<tm2.1Mym>
Former Common nameTrsp<del>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Dr. Masayuki Yamamoto, Center for Tsukuba Advanced Research Alliance, University of Tsukuba in 2008. Trsp<tm2.1Mym> allele was produced by the crossing Trsp floxed (Trsp<tm2Mym>) (RBRC02681) and AyuI-Cre mice.
Strain descriptionTrsp knockout mice. Trsp gene codes Selenocysteine-tRNA which is essential for the synthesis of selenium containing protein. Inactivation of this gene induces disruption of the synthesis of selenium containing protein. Homozyous mutant mice are embryonic lethal.
Colony maintenance
ReferencesJ. Biol. Chem., 283, 2021-2030 (2008) 18039655

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TrsptRNA phosphorerine7Trsp<tm2.1Mym>targeted mutation 2.1, Masayuki Yamamotoopal suppressor, selenocysteine tRNA, tRNA Sec

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAmouse Trsp genomic DNA, phage P1 loxP
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Suzuki et al., J. Biol. Chem., 283, 2021-2030 (2008).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorMasayuki Yamamoto (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data