Strain Data Sheet

RBRC02698

Strain Information

Image
BRC No.RBRC02698
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rapgef2<tm1.1Tkat>
Former Common nameRA-GEF-1<+/->
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strainC57BL/6 (semicongenic)
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. The mice were crossed with Tg(ACTB-cre)13Miya mice. C57BL/6 semicongenic.
Strain descriptionRA-GEF-1 (Rapgef2) gene knockout mice. Exon 15 was removed. Homozygous knockout mice are embryonic lethal due to an abnormal vascular development.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesBiochem. Biophys. Res. Commun., 363, 106-112 (2007). 17826737

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rapgef2Rap guanine nucleotide exchange factor (GEF) 23Rapgef2<tm1.1Tkat>targeted mutation 1.1, Tohru Kataoka5830453M24Rik, CNRasGEF, mKIAA0313, nRapGEP, Pdzgef1, RA-GEF-1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Rapgef2 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biochem. Biophys. Res. Commun., 363, 106-112 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.
DepositorTohru Kataoka (Kobe University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data