Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC02700  
Type Targeted Mutation CongenicCartagena   wks
Species Mus musculus  
Strain name B6.Cg-Rapgef2<tm1Tkat> Rapgef6<tm1.1Tkat>  
Former Common name RA-GEF-1, RA-GEF-2<-/->  
H-2 Haplotype No Data  
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Tohru Kataoka, Kobe University Graduate School of Medicine in 2007. E14TG2a ES cells were used to generate the mutant mice. RA-GEF-2 (Rapgef6) knockout allele was generated by crossing with Rapgef6 floxed and Tg(ACTB-cre)13Miya mice. C57BL/6 semicongenic.  
Strain description RA-GEF-1 (Rapgef2) floxed and RA-GEF-2 (Rapgef6) knockout double mutant mice. Exon 15 of the RA-GEF-1 (Rapgef2) gene was flanked by loxP sites (Homozygous mice are viable and fertile). Exon 21 of the RA-GEF-2 (Rapgef6) gene was removed (homozygous knockout mice are viable and fertile, but exhibit an inlarged spleen, increased IgE and IgG levels and altered cytokine production).  
Colony maintenance Rapgef2: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]; Rapgef6: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Rapgef6  
Symbol name Rap guanine nucleotide exchange factor (GEF) 6  
Chromosome 11  
Common name C030018K18Rik, PDZ-GEF2, Pdzgef2, RA-GEF-2  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 11  
Common name No Data  
Symbol description No Data  
 3 Symbol Rapgef2  
Symbol name Rap guanine nucleotide exchange factor (GEF) 2  
Chromosome 3  
Common name 5830453M24Rik, CNRasGEF, mKIAA0313, nRapGEP, Pdzgef1, RA-GEF-1  
Symbol description No Data  
 4 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 3  
Common name No Data  
Symbol description No Data  
References Biochem Biophys Res Commun. 2007 Sep 7;.
Mol Biol Cell. 2007 May 30;.  
Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1.
Defective vascular morphogenesis and mid-gestation embryonic death in mice lacking RA-GEF-1.
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 363, 106-112 (2007). Mol. Biol. Cell, 18, 2949-2959 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Kataoka, Tohru (Kobe University)  Kataoka, Tohru 
Strain Status /
Availability
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