Strain Data Sheet

RBRC02704

Strain Information

Image
BRC No.RBRC02704
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(FucciS/G2/M)#474Bsi
Former Common nameB6.Cg-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain descriptionTransgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenanceCarrier x Carrier [or Crossing to C57BL/6NJcl]
ReferencesCell, 132, 487-498 (2008). 18267078
Development, 140(22):4624-32 (2013) doi:10.1242/dev.099226 24154524
PLoS One, 8(9):e73801 (2013). 24066072

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:A
2023/10/23Room:3-CRack:A
2023/07/24Room:3-CRack:A
2023/04/24Room:3-CRack:A
2023/01/23Room:3-CRack:A
2022/10/25Room:3-CRack:A
2022/08/22Room:3-CRack:A
2022/07/25Room:3-CRack:A
2022/04/25Room:3-CRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GMNNgeminin (human)UNGMNN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mAGmonomeric Azami Green (Coral)UNmAGCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Coral mAG(monomeric Azami Green) cDNA, human Geminin cDNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLOS ONE (2013) doi: 10.1371/journal.pone.0073801. Development (2013) doi:10.1242/dev.099226.The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki <FAX: +81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp> of Osaka University.
DepositorAtsushi Miyawaki (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genetic Background
Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.
Fucci-guided purification of hematopoietic stem cells with high repopulating activity.
Biochem Biophys Res Commun 457(1) 7-11(2015) 25534850

Thion MS, Low D, Silvin A, Chen J, Grisel P, Schulte-Schrepping J, Blecher R, Ulas T, Squarzoni P, Hoeffel G, Coulpier F, Siopi E, David FS, Scholz C, Shihui F, Lum J, Amoyo AA, Larbi A, Poidinger M, Buttgereit A, Lledo PM, Greter M, Chan JKY, Amit I, Beyer M, Schultze JL, Schlitzer A, Pettersson S, Ginhoux F, Garel S.
Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner.
Cell 172(3) 500-516.e16(2018) 29275859

Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG.
Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions.
Immunity 48(2) 364-379.e8(2018) 29466759

Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.
Involvement of Receptor Activator of Nuclear Factor-κB Ligand (RANKL)-induced Incomplete Cytokinesis in the Polyploidization of Osteoclasts.
J Biol Chem 291(7) 3439-54(2016) 26670608

Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K.
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes.
Proc Natl Acad Sci U S A 111(21) 7771-6(2014) 24825888

Teh YC, Chooi MY, Liu D, Kwok I, Lai GC, Ayub Ow Yong L, Ng M, Li JLY, Tan Y, Evrard M, Tan L, Liong KH, Leong K, Goh CC, Chan AYJ, Shadan NB, Mantri CK, Hwang YY, Cheng H, Cheng T, Yu W, Tey HL, Larbi A, St John A, Angeli V, Ruedl C, Lee B, Ginhoux F, Chen SL, Ng LG, Ding JL, Chong SZ.
Transitional premonocytes emerge in the periphery for host defense against bacterial infections.
Sci Adv 8(9) eabj4641(2022) 35245124