Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC02759  
Type Targeted Mutation CongenicCartagena  
Species Mus musculus  
Strain name B6.Cg-Atg7<tm1Tchi>  
Former Common name C57BL/6-Atg7 flox/flox  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance black  
Genotype a/a B/B C/C  
Strain development Developed by Masaaki Komatsu and Tomoyuki Chiba, Tokyo Metropolitan Institute of Medical Science. The targeting construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mice were backcrossed to C57BL/6 over 5 times.  
Strain description Atg7, autophagy-related 7 gene floxed mice. A targeting vector was constructed by insertion of a loxP site within introns 13 and 14. Exon 14 was fused to a cDNA fragment encoded by exons 15, 16 and 17 and polyA after the stop codon. Atg7 is essential for ATG conjugation, LC3 modification systems, and autophagosome formation. Conditional Atg7 deficient mice can be generated by crossing with tissue-specific Cre mice.  
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Atg7  
Symbol name autophagy-related 7 (yeast)  
Chromosome 6  
Common name 1810013K23Rik, Apg7l  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 6  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 6  
Common name neo; neomycin;  
Symbol description No Data  
References J Cell Biol 2005 May 9;169(3):425-34. Epub 2005 May 2.  
Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice.
Research applications No Data  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Cell Blol., 169, 425-434 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE, Who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents use with the results from the use of the BIOLOGICAL RESOURCE.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Jun 2012, Mouse of the Month Oct 2016  
 3 Genotyping protocol <PCR>  
Depositor Komatsu, Masaaki (Tokyo Metropolitan Institute of Medical Science)  Komatsu, Masaaki 
Strain Status /
Availability
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