Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC02794  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Vps52<tm1Kab>/95Rbrc  
Former Common name Vps52 #95(Acc.No.CDB0767K)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).  
Strain description Vps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).  
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Vps52  
Symbol name VPS52 GARP complex subunit  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 2 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 3 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 4 Symbol HBB2  
Symbol name rabbit beta-globin  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 5 Symbol lox71  
Symbol name mutant lox sequence  
Chromosome 17  
Common name No Data  
Symbol description No Data  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 17  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 7 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 17  
Common name neo; neomycin;  
Symbol description No Data  
References   
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (http://www.cdb.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.  
Additional information
 1 Lab HP  
 2 Genotyping protocol <PCR>  
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology)  RIKEN, CDB 
Strain Status /
Availability
(Expected delivery)

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