Strain Information | |
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Image | |
BRC No. | RBRC02794 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Vps52<tm1Kab>/95Rbrc |
Former Common name | Vps52 #95(Acc.No.CDB0767K) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic). |
Strain description | Vps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857). |
Colony maintenance | Heterozygote x Wild-type [C57BL/6NCrlCrlj] |
References | Cell Rep., 2(5) 1363-74 (2012). 23142660 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site17Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin17HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse PGK 3’end, rabbit beta-globin 3' end, mouse Vps52 genomic DNA |
Research application | Cre/loxP system Developmental Biology Research FLP/frt system |
Specific Term and Conditions | (1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (https://large.riken.jp/distribution/mutant-list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |