Strain Data Sheet

RBRC02838

Strain Information

Image
BRC No.RBRC02838
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(Camk2a-GFP/DA)15
Former Common nameGFP-DATg
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrlCrlj
Appearance
Strain developmentDeveloped by Tomoaki Shirao, Gunma University School of Medicine in 2001.
Strain descriptionDrebrin is an F-actin-binding protein that is highly expressed in the brain and regulates the dynamics of actin cytoskeletal networks. These mice specifically overexpress a fusion protein of rat drebrin A and GFP in the forebrain. A western blot analysis of cerebral cortex extract revealed a 10 to 15% increase in drebrin (similar expression patterns were observed in three lines: RBRC02838, RBRC02839, RBRC0284015). These mice are useful for analyzing the effect of the overexpression of drebrin at the cellular and body levels.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dbn1drebrin 1 (rat)UNDbn1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UNmouse Camk2a promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAオワンクラゲGFP cDNA, ラット drebrin A cDNA, SV40 polyA signal, mouse Camk2a genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT must contact the DEPOSITOR/DEVELOPER to obtain permission for use o the BIOLOGICAL RESOURCE as a collaboration research.
DepositorTomoaki Shirao (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data