Strain Information | |
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Image | |
BRC No. | RBRC03155 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | Pcdhg-gHS4-5(133) |
Former Common name | gHS4-5(133) |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. TT2 ES cells were used. The gHS4-5 dNeo mice were generated by crossing with gHS4-5 (RBRC02811) and FLP mice. |
Strain description | Pcdhg gene floxed mice. The gHS4-5 genomic region of the Pcdhg gene was flanked by loxP sites. Frt-neo cassette type (RBRC02811 and RBRC03155), delta neo cassette type (RBRC03153). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)] |
References | J Biol Chem. , 286, 31885-31895 (2011). 21771796 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site18Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP18loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)18mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | phage P1 loxP, yeast FRT (flipase recombination target) site, Transposon Tn5 Neo, mouse phosphoglycerate kinase promoter (PGK promoter), Mouse Pcdhg gene |
Research application | Cre/loxP system FLP/frt system Neurobiology Research |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR. |
Depositor | Takeshi Yagi (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |