Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC03513  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Isx<tm1Sse>/Rbrc  
Former Common name Isx(lacZ) (Acc.No. CDB0475K)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Susumu Seino, Kobe University Graduate School of Medicine. TT2 ES cells were used to generate the mutant mice.  
Strain description Isx gene knockout mice. Exon1 was replaced with a lacZ-neo cassette.  
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Frt  
Symbol name yeast FRT (flippase recombination target) site  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 2 Symbol HBB2  
Symbol name rabbit beta-globin poly A  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 3 Symbol Isx  
Symbol name intestine specific homeobox  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 4 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 5 Symbol lox71  
Symbol name mutant lox sequence  
Chromosome 8  
Common name No Data  
Symbol description No Data  
 6 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 8  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 7 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 8  
Common name No Data  
Symbol description No Data  
References J Biol Chem. 2008 Feb 22;283(8):4905-11. Epub 2007 Dec 19.  
Isx participates in the maintenance of vitamin A metabolism by regulation of beta-carotene 15,15'-monooxygenase (Bcmo1) expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Susumu Seino (seino@med.kobe-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0475K) (http:www.cdb.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Biol Chem. 2008 283(8):4905-11.) (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.  
Additional information
 1 No Data  
 2 Genotyping protocol <PCR>  
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology)  RIKEN, CDB 
Strain Status /
Availability
(Expected delivery)

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