Strain Data Sheet

RBRC03514

Strain Information

Image
BRC No.RBRC03514
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hgs<tm>/Rbrc
Former Common nameHrs(loxP)(Acc.No. CDB0476K)
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuo Sugamura, Tohoku University Graduate School of Medicine in 2008. Deposited by Laboratory for Animal Resrouces and Genetic Engineering, RIKEN Center for Developmental Biology.
Strain descriptionHrs (Hgs, HGF-regulated tryosine kinase substrate) floxed mice. Conditional Hrs deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance
ReferencesAm. J. Pathol., 173, 1806-1817 (2008). 19008375

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HgsHGF-regulated tryosine kinase substrate11Hgs<tm>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli neo, Phage P1 loxP sites, yeast FRT (flipase recombination target) sites, mouse Hgs genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and Conditions(1) The RECIPIENT shall obtain a prior agreement from Nobuyuki Tanaka (tanaka@med.tohoku.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0476K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Am J Pathol. 2008 173(6):1806-17.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorRIKEN CDB (RIKEN CDB)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data