Strain Data Sheet
ANIMAL SEARCH SYSTEM
Data update: Jun 20, 2019
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RBRC No. RBRC03515  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.Cg-Aire<tm2Mmat>/Rbrc  
Former Common name Aire(GFP) (Acc.No. CDB0479K)  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology and Mitsuru Matsumoto, Institute for Enzyme Research, University of Tokushima. TT2 ES cells were used.  
Strain description Aire GFP knock-in mice. A GFP was inserted in the Aire gene locus between exon 1 and 2.  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Aire  
Symbol name autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 2 Symbol GFP  
Symbol name Green Fluorescent Protein (Jellyfish)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 10  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 10  
Common name No Data  
Symbol description No Data  
References J Exp Med. 2008 Nov 24;205(12):2827-38. Epub 2008 Nov 17.  
Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Mitsuru Matsumoto (mitsuru@ier.tokushima-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0479K) (http:www.cdb.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Exp Med. 2008 205(12):2827-38.) (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.  
Additional information
 1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 2 Genotyping protocol <PCR>  
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology)  RIKEN, CDB 
Strain Status /
Availability
(Expected delivery)

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