Strain Information | |
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Image | |
BRC No. | RBRC03519 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6D2;129P2-Cthrc1<tm1Hssk>/Rbrc |
Former Common name | Cthrc1(lacZ) (Acc.No. CDB0502K) |
H-2 Haplotype | |
ES Cell line | E14TG2a [129P2/OlaHsd] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroshi Sasaki, RIKEN Center for Developmental Biology. A lacZ and PGK-neo cassette was transferred into E14TG2a ES cells to replace the exon 1 of Cthrc1 gene. |
Strain description | Cthrc1 gene knockout mice. Cthrc1 is a Wnt signaling component that selectively activates the PCP pathway. |
Colony maintenance | |
References | Dev. Cell., 15, 23-36 (2008). 18606138 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1Hssk>targeted mutation 1, Hiroshi Ssasaki Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin 3' end15HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)15 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lox71mutant lox sequence15lox71 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)15 |
Ordering Information | |
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Donor DNA | Neo : E. coli, lacZ : E. coli, lox71 and loxP : P1 Phage, frt : yeast FRT (flipase recombination target) sites, PGK promoter and PGK 3' end : mouse, rabbit beta-globin 3' end : Rabbit |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0502K) (https://large.riken.jp/distribution/mutant-list.html) and the BIOLOGICAL RESOURCE is requested. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Cell. 2008 15(1):23-36.) (3) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published. |
Depositor | RIKEN CDB (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Li J, Wang Y, Ma M, Jiang S, Zhang X, Zhang Y, Yang X, Xu C, Tian G, Li Q, Wang Y, Zhu L, Nie H, Feng M, Xia Q, Gu J, Xu Q, Zhang Z. Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-β signaling. EBioMedicine 40 43-55(2019) 30639416 |