Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC03556  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6.129-Dmd<tm1Mok>  
Former Common name Mdx52, B6-Dmd KO  
H-2 Haplotype No Data  
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Motoya Katsuki, Institute of Medical Science, University of Tokyo. CCE/EK.CCE ES cells were used. The mutant mice were backcrossed to C57BL/6 by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.  
Strain description dystrophin (Dmd) gene knockout mice. The exon 52 was replaced with a neo cassette. Mutant mice show hypertrophic skeletal muscles. Homozygous female and hemizygous male mutants are viable and fertile. The mice are difficult to start breeding at 8-10 weeks (12-16 weeks is better).  
Colony maintenance Homozygote (female) x Hemizygote (male) [or Crossing to C57BL/6JJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Dmd  
Symbol name dystrophin, muscular dystrophy  
Chromosome X  
Common name No Data  
Symbol description No Data  
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)  
 2 Symbol neo  
Symbol name neomycin resistance gene (Klebsiella pneumoniae)  
Chromosome X  
Common name No Data  
Symbol description No Data  
References Biochem Biophys Res Commun. 1997 Sep 18;238(2):492-7.  
Targeted disruption of exon 52 in the mouse dystrophin gene induced muscle degeneration similar to that observed in Duchenne muscular dystrophy.
Research applications Mouse Models for Human Disease  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 238, 492-497 (1997).  
Additional information
 1 Mouse of the Month Apr 2014  
 2 Genotyping protocol <PCR>  
Depositor Hashimoto, Naohiro (National Institute for Longevity Sciences)  Hashimoto, Naohiro 
Strain Status /
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