Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC03559  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name 129.Cg-Pax7<tm2Pgr>  
Former Common name PaxZ129, 129-Pax7/lacZ  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Peter Gruss, Max Planck Institute for Biophysical Chemistry. The mutant mice were backcrossed 129 over 20 times by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.  
Strain description Pax7 gene knockout mice. The first exon of the paired box was replaced with a neo cassette (RBRC03558) and lacZ-neo cassette (RBRC03559), respectively. In the C57BL/6 background, most homozygous mutant mice die within 3 weeks after birth (survival rate is below 10%). On the other hand, in the 129 background (RBRC03558 and RBRC03559), approximately 50% of mutant mice die within 3 weeks after birth (details unknown). Heterozygous mutant mice for lacZ repoter Pax7<tm2Pgr> (RBRC03559) show negative X-gal staining in the muscle satellite cells.  
Colony maintenance Heterozygote x Wild-type [or Crossing to 129<+Ter>/SvJcl]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol Pax7  
Symbol name paired box gene 7  
Chromosome 4  
Common name No Data  
Symbol description No Data  
 2 Symbol lacZ  
Symbol name beta-galactosidase (E. coli)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 3 Symbol neo  
Symbol name neomycin resistance gene (Klebsiella pneumoniae)  
Chromosome 4  
Common name No Data  
Symbol description No Data  
References
  

Research applications Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Pax7tm2Pgr allele (Peter Gruss, Max Planck Institute for Biophysical Chemistry). (http://www.mpibpc.mpg.de/de/gruss)  
Additional information
 1 MATERIAL TRANSFER AGREEMENT (English)  
 2 Genotyping protocol <PCR>  
Depositor Hashimoto, Naohiro (National Institute for Longevity Sciences)  Hashimoto, Naohiro 
Strain Status /
Availability
(Expected delivery)

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