Strain Data Sheet

RBRC03559

Strain Information

Image
BRC No.RBRC03559
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129.Cg-Pax7<tm2Pgr>
Former Common namePaxZ129, 129-Pax7/lacZ
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Peter Gruss, Max Planck Institute for Biophysical Chemistry. The mutant mice were backcrossed 129 over 20 times by Naohiro Hashimoto, National Center for Geriatrics and Gerontology.
Strain descriptionPax7 gene knockout mice. The first exon of the paired box was replaced with a neo cassette (RBRC03558) and lacZ-neo cassette (RBRC03559), respectively. In the C57BL/6 background, most homozygous mutant mice die within 3 weeks after birth (survival rate is below 10%). On the other hand, in the 129 background (RBRC03558 and RBRC03559), approximately 50% of mutant mice die within 3 weeks after birth (details unknown). Heterozygous mutant mice for lacZ repoter Pax7<tm2Pgr> (RBRC03559) show negative X-gal staining in the muscle satellite cells.
Colony maintenanceHeterozygote x Wild-type [or Crossing to 129<+Ter>/SvJcl]
ReferencesCell, 102, 777-786 (2000). 11030621
Development, 122, 831-838 (1996). 8631261

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pax7paired box gene 74Pax7<tm2Pgr>targeted mutation 2, Peter Gruss

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (Klebsiella pneumoniae)4mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Beta-galactosidase, Klebsiella pneumoniae neomycin resistance gene, bovine growth hormone polyadenylation signal, mouse Pax7 genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Pax7tm2Pgr allele (Peter Gruss, Max Planck Institute for Biophysical Chemistry). (https://www.oist.jp/peter-gruss)
DepositorNaohiro Hashimoto (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. MATERIAL TRANSFER AGREEMENT (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data