Strain Data Sheet
Data update: Mar 22, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC03674  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name ICR.Cg-Gad1<tm1.1Tama>  
Former Common name GAD67-GFP dNeo KI  
H-2 Haplotype No Data  
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Yuchio Yanagawa, Graduate School of Medicine, Gumma University. ICR mixed background.  
Strain description An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are neonatal lethal due to cleft palate. A loxP flanked neo was removed by crossing with cre mice.  
Colony maintenance Heterozygote x Wild-type [or Crossing to Jcl:MCH(ICR)]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol EGFP  
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 2 Symbol Gad1  
Symbol name glutamate decarboxylase 1  
Chromosome 2  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 2  
Common name No Data  
Symbol description No Data  
References J Comp Neurol 2003 Dec 1;467(1):60-79.  
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Comp Neurol., 467, 60-79 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The BIOLOGICAL RESOURCE should not be redistributed without DEPOSITOT's permission. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.  
Additional information
 1 Mouse of the Month Jan 2014  
 2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.  
 3 Genotyping protocol <PCR>  
Depositor Yanagawa, Yuchio (Gunma university)  Yanagawa, Yuchio 
Strain Status /
(Expected delivery)

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