Strain Data Sheet
RBRC03705
Strain Information | |
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| Image | |
| BRC No. | RBRC03705 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6N-Tg(Nes-cre)52Utr/Rbrc |
| Former Common name | Nestin-NLSCre |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6NCrlCrlj |
| Appearance | |
| Strain development | Developed by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background. |
| Strain description | Cre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse nestin promoter fragment derived from the BAC library (BAC clone RP24-64E17)was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community. |
| Colony maintenance | Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsmouse Nestin promoter (neuronal-specific promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN |
Ordering Information | |
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| Donor DNA | Cre recombinase [P1_ap003, Bacteriophage genomic DNA (class 1)], FRT sequence [yeast genomic DNA (class 1)], Nestin [mouse genomic DNA (neuronal-specific promoter)(class 1)], Phage P1 loxP derived from pBACe3.6 vector, BAC clone RP23-141A13, SV40 poly A, SV40 Large T antigen nuclear localization signal (NLS) |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | RIKEN BRC (RIKEN BRC) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |