Strain Data Sheet

RBRC03899

Strain Information

Image
BRC No.RBRC03899
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameSTOCK Ephb1<tm1Cmn> Ephb2<tm1Paw> Ephb3<tm1Kln> EphB6<tm2Tom>
Former Common nameEphB1, EphB2, EphB3, EphB6 fourfold knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshimitsu Mastui, Kobe University Graduate School of Medicine. 129 and ICR mixed background.EphB1, EphB2, EphB3 KO: Mark Henkemeyer, University of Texas Southwestern Medical Center; EphB6 KO: Hiroshi Suzuki and Toshimitsu Mastui, Kobe University.
Strain description
Colony maintenance
ReferencesBiochem. Biophys. Res. Commun., 298, 87-94 (2002). 12379224
EMBO J., 15, 6035-6049 (1996). 8947026
J. Cell. Biol., 163, 1313-1326 (2003). 14691139
Eur. J. Immunol., 42, 1562-1572 (2012). 22622783
Cell, 86, 35-46, (1996). 8689685
Neuron., 39, 919-935 (2003). 12971893

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ephb3Eph receptor B316Ephb3<tm1Kln>targeted mutation 1, Rudiger KleinSek4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)16neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ephb2Eph receptor B24Ephb2<tm1Paw>targeted mutation 1, Tony PawsonNuk

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ephb6Eph receptor B66Ephb6<tm2Tom>targeted mutation 2, Toshimitsu Matsui

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ephb1Eph receptor B19Ephb1<tm1Cm>targeted mutation 1, Carol A Mason

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9neo; neomycin;

Ordering Information

Donor DNAmouse Phosphoglycerate kinase (PGK) promoter, E. coli neo, Phage P1 loxP sites, mouse Ephb1 genomic DNA, mouse Ephb2 genomic DNA, mouse Ephb3 genomic DNA, mouse Ephb6 genomic DNA
Research applicationCell Biology Research
Cre/loxP system
Neurobiology Research
Specific Term and Conditions1. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures designated by use of the DEPOSITOR is requested. Henkemeyer et al., 1996 paper in Cell for EphB2/EphB3, Williams et al., 2003 paper in Neuron for EphB1. Shimoyama et al., 2002 paper in BBRC for EphB6, or Kawano et. al., 2012 paper in Eur. J. Immunol. for EphB1/EphB2/EphB3/EphB6 quadruple mutant mice.
2. UT Southwestern would like to limit the use of the BIOLOGICAL RESOURCE to non-profit institutions, also limit the use to academic research purposes and for-profit institutions or use for commercial research purposes shall acquire a prior written consent from the Depositor, a recipient shall acquire a prior written consent form the Depositor prior to filing an application for a patent, or intellectual property or other right based on the results research using the BIOLOGICAL RESOURCE.
DepositorDrs. Toshimitsu Matsui and Mark Henkemeyer (Kobe University/ University of Texas Southwestern Medical Center)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data