Strain Information | |
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Image | |
BRC No. | RBRC03934 |
Type | Transgene |
Species | Mus musculus |
Strain name | C57BL/6N-Tg(Ins1-cre)25Utr/Rbrc |
Former Common name | Insulin-NLSCre |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NCrlCrlj |
Appearance | |
Strain development | Developed by Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResrouce Center in 2009. The transgene was injected into the pronuclei of C57BL/6N fertilized eggs. |
Strain description | BAC Ins1-Cre transgenic mice. The mice were generated by using a BAC clone containing the mouse Ins1 gene, PR23-181I21. The Cre gene was inserted by homologous recombination into the second exon with a translational initiation codon in the Ins1 BAC clone. 6 founder lines were generated. The R26R crossing test indicated that line 25 (RBRC03934) is useful cre-driver for pancreatic beta cell-specific Cre-loxP recombination. |
Colony maintenance | Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown). |
References | Exp. Anim., 63(2):183-191 (2014). 24770644 Exp. Anim., 62, 295-304 (2013). 24172193 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteUNFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinaseUNcre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPUNloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter nlsSV40 large T antigen nuclear localization signal (NLS)UNnlsmouse Insulin promoter (beta cell specific promoter region) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyAUN |
Ordering Information | |
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Donor DNA | Phage P1 cre recombinase, yeast FRT (flippase recombination target) site, mouse Insulin promoter (beta cell specific promoter region), SV40 polyA, SV40 nuclear location signal(nls), Phage P1 loxP site derived from pBACe3.6 vector, BAC clone RP23-181I21 |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 63(2):183-191 (2014). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | RIKEN BRC (RIKEN BRC) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Ueki K, Nishida Y, Aoyama S, Uzawa H, Kanai A, Ito M, Ikeda K, Iida H, Miyatsuka T, Watada H. Establishment of Pancreatic Beta Cell-specific Gene Knockout System Based on CRISPR-Cas9 Technology with AAV8-mediated gRNA Delivery. Diabetes (2023) 37625131 |