Strain Information | |
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Image | |
BRC No. | RBRC03977 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Chr5-Pirc<tm1Ms> |
Former Common name | Chr5 piRNA cluster KO |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Toshiaki Watabe and Yasuhiro Yamamoto, National Institute of Genetics in 2009. R1 ES cells were used. |
Strain description | Chromosome 5 piRNA cluster knockout mice. The promoter region of RNA precursor was replaced with a neo cassette. piRNA from Chr5 piRNA cluster was not generated. So far homozygous mutant mice show no obvious abnormality. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Chr5-PircChromosome 5 piRNA cluster5Chr5-Pirc<tm1Ms>targeted mutation 1, National Institute of Genetics Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)5GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP5loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | E. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, bovine Growth hormone polyadenylation signal, P1 phage loxP |
Research application | Cell Biology Research Cre/loxP system Reproductive Biology Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For two years form deposit, the RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR and DEVELOPER. |
Depositor | Hiroyuki Sasaki (Kyushu University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |