Strain Data Sheet

RBRC03977

Strain Information

Image
BRC No.RBRC03977
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Chr5-Pirc<tm1Ms>
Former Common nameChr5 piRNA cluster KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Toshiaki Watabe and Yasuhiro Yamamoto, National Institute of Genetics in 2009. R1 ES cells were used.
Strain descriptionChromosome 5 piRNA cluster knockout mice. The promoter region of RNA precursor was replaced with a neo cassette. piRNA from Chr5 piRNA cluster was not generated. So far homozygous mutant mice show no obvious abnormality.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Chr5-PircChromosome 5 piRNA cluster5Chr5-Pirc<tm1Ms>targeted mutation 1, National Institute of Genetics

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)5GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE. coli Neomycin resistance gene, Mouse Phosphoglycerate kinase promoter, bovine Growth hormone polyadenylation signal, P1 phage loxP
Research applicationCell Biology Research
Cre/loxP system
Reproductive Biology Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and DEVELOPER in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For two years form deposit, the RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR and DEVELOPER.
DepositorHiroyuki Sasaki (Kyushu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data