Strain Information | |
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Image | |
BRC No. | RBRC04109 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S6-Calca<tm1Hku> |
Former Common name | alphaCGRP- |
H-2 Haplotype | |
ES Cell line | SM1[129S6/SvEvTac] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroki Kurihara, Graduate School of Medicine, University of Tokyo in 2001. |
Strain description | The alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT. The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability. |
Colony maintenance | The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability. |
References | Circ. Res., 89, 983-990 (2001). 11717154 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Calca genomic DNA |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ res., 89, 983-990 (2001).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
Depositor | Hiroki Kurihara (The University of Tokyo) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- Genetic Background |
BRC mice in Publications |
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Jiang Y, Yuan Y, Xiong Y, Wang B, Guo Y, Gong P, Zhang L. Low-intensity pulsed ultrasound improves osseointegration of dental implant in mice by inducing local neuronal production of αCGRP. Arch Oral Biol 115 104736(2020) 32371135 Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P. Effect of lentiviral vector overexpression α-calcitonin gene-related peptide on titanium implant osseointegration in α-CGRP-deficient mice. Bone 94 135-140(2017) 26265538 Wang B, Lin J, Zhang Q, Zhang X, Yu H, Gong P, Xiang L. αCGRP Affects BMSCs' Migration and Osteogenesis via the Hippo-YAP Pathway. Cell Transplant 28(11) 1420-1431(2019) 31426665 |