Strain Data Sheet
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Data update: Jun 20, 2019
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RBRC No. RBRC04292  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6-Pold1<tm1.uchi>  
Former Common name Pold1  
H-2 Haplotype No Data  
Background strain No Data  
Appearance
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The mutant mice were crossed to C57BL/6J.  
Strain description Pold1 (polymerase (DNA directed), delta 1, catalytic subunit) gene knockout mice. A loxP flanked polyA-neo cassette was inserted in intron 10. The exon 10 contains a D400A substitution. Homozygous mutant mice are embryonic lethal.  
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]  
Health Report
Gene Details
Promoter No Data  
 1 Symbol No Data  
Symbol name SV40 PolyA signal  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 2 Symbol Pold1  
Symbol name polymerase (DNA directed), delta 1, catalytic subunit  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 3 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 7  
Common name No Data  
Symbol description No Data  
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)  
 4 Symbol neo  
Symbol name neomycin resistance gene (E. coli)  
Chromosome 7  
Common name No Data  
Symbol description No Data  
References   
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.  
Additional information
 1 Lab HP (Japanese)  
 2 Genotyping protocol <PCR>  
Depositor Yagi, Takeshi (Graduate School of Frontier Biosciences, Osaka University)  Yagi, Takeshi 
Strain Status /
Availability
(Expected delivery)

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