Strain Data Sheet
Data update: Jun 20, 2019
Health Report   PCR Protocol   Thawing Method
embryos / sperm >
  Mouse Marking
RBRC No. RBRC04293  
Type Targeted MutationCartagena  
Species Mus musculus  
Strain name B6-Pold1<exo.uchi>  
Former Common name Pold1  
H-2 Haplotype No Data  
ES cell line Not specified (ES cell)  
Background strain No Data  
 1 Appearance No Data  
Genotype No Data  
Strain development Developed by Arikuni Uchimura and Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University in 2007. C57BL/6J derived ES cells were used to generate the knockout mice. The polyA-neo cassette was removed by crossing with Sycp1-cre mice. The mutant mice were crossed to C57BL/6J.  
Strain description Pold1 (polymerase (DNA directed) D400A variant mutant mice lacking 3'-5'exonuclease activity in the Pol delta. About 30% of homozygous mutant mice die with abnormally swollen thymus. Most surviving mice develop nodules on their tails. It is preferable to avoid a mating using heterozygous male across continuous generations (2 generations and up) to avoid an increasing mutation rate.  
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]  
Health Report No Data  
Gene Details
Promoter No Data  
 1 Symbol Pold1  
Symbol name polymerase (DNA directed), delta 1, catalytic subunit  
Chromosome 7  
Common name No Data  
Symbol description No Data  
 2 Symbol loxP  
Symbol name phage P1 loxP  
Chromosome 7  
Common name No Data  
Symbol description No Data  
Research applications Cell Biology Research,
Cre/loxP system  
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.  
Additional information
 1 Lab HP (Japanese)  
 2 Mouse of the Month Dec 2015  
 3 Genotyping protocol <PCR>  
Depositor Yagi, Takeshi (Graduate School of Frontier Biosciences, Osaka University)  Yagi, Takeshi 
Strain Status /
(Expected delivery)

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