Strain Data Sheet

RBRC04323

Strain Information

Image
BRC No.RBRC04323
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6C-Tg(CAG-EGFP/miR21)
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Takashi Iwamoto, Department of Biomedical Sciences, Chubu Universiy in 2009.
Strain descriptionmiR-21 is a potent tumor promoting gene and may be involved in cardiac hypertrophy. Their expression can be monitored by GFP.
Colony maintenance
ReferencesCancer Res., 65, 6029-6033 (2005). 16024602
Nature, 456, 980-984 (2008). 19043405

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
MIR21miR-21UNMIR21

Ordering Information

Donor DNAchicken Beta actin promoter, cytomergarovirus CMV enhancer, Homo sapiens pri-miR-21, Rabbit Globin PolyA signal, Aequrea Victoria EGFP
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakashi Iwamoto (Chubu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data