Strain Data Sheet

RBRC04325

Strain Information

Image
BRC No.RBRC04325
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain name129S2.129(B6)-Crym<tm1Sasu>
Former Common nameCRYM -/- mice
H-2 Haplotype
ES Cell line
Background strain129S2/SvPas
Appearance
Strain developmentDeveloped by Satoru Suzuki, Institute on Aging and Adaptation, Shinshu University in 2004. The mutant mice were backcrossed to 129S2/SvPasCrl over 10 times.
Strain description
Colony maintenance
ReferencesMol. Cell Biol., 21, 4748-4760 (2001). 11416150
Mol. Endocrinol., 21, 885-894 (2007). 17264173

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Crymcrystallin, mu7Crym<tm1Sasu>targeted mutation 1, Satoru Suzuki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Crym genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Endocrinol., 21, 885-894 (2007).
DepositorSatoru Suzuki (Shinshu University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data