Strain Data Sheet
RBRC04437
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC04437 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | ICR.129(B6)-Msi2<tm1Okn> |
| Former Common name | |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shinsuke Shibata and Hideyuki Okano, Keio University School of Medicine. |
| Strain description | Msi2 deficient mice (RBRC04436 and RBRC00437) survive to adult and show poor weight gain, low spontaneous movement and sensory dysfunction, with abnormal PNS development. The cause of the phenotype is thought to be poor neural fiber innervations of specific sensory neuron from DRG to spinal cord. These phenotypes are observed both background (C57BL/6J and ICR) and detailed analyses were performed in ICR background. Msi2 deficient male mice show azoospermia and are infertile. Because the movement of sperm taken from epidermis of Msi2 null male was bad, in vitro fertilization was not successful. Msi2 deficient female mice are fertile; however, probability of pregnancy is poor presumably due to the low spontaneous activity. |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP11loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)11neo; neomycin; |
Ordering Information | |
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| Donor DNA | mouse Musashi2 genomic DNA, E.coli neo, mouse phosphoglycerate kinase promoter (PGK promoter), Mouse Phosphoglycerate kinase PolyA signal, phage P1 loxP |
| Research application | |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) For use of the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Hideyuki Okano (Keio University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |