Strain Information | |
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Image | |
BRC No. | RBRC04549 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S2-Nmu<tm1Mko> |
Former Common name | NMU-KO |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6. |
Strain description | Nmu (Neuromedin U) gene knock-out mice. Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms gene knockout mice (RBRC04550), Nmu and Nms double knockout mice (RBRC04737). |
Colony maintenance | Heterozygote × wild-type [or Crossing to C57BL/6JJcl] |
References | J. Exp. Med., 202, 217-224 (2005). 16009716 Nat. Med., 10, 1067-1073 (2004). 15448684 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-3Rack:B 2023/12/11Room:3-3Rack:B 2023/09/11Room:3-3Rack:B 2023/06/13Room:3-3Rack:B 2023/03/13Room:3-3Rack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA |
Research application | Metabolism Research |
Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Msayasu Kojima (Kurume University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (3 to 6 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Wallrapp A, Riesenfeld SJ, Burkett PR, Abdulnour RE, Nyman J, Dionne D, Hofree M, Cuoco MS, Rodman C, Farouq D, Haas BJ, Tickle TL, Trombetta JJ, Baral P, Klose CSN, Mahlakõiv T, Artis D, Rozenblatt-Rosen O, Chiu IM, Levy BD, Kowalczyk MS, Regev A, Kuchroo VK. The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation. Nature 549(7672) 351-356(2017) 28902842 |