Strain Information | |
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Image | |
BRC No. | RBRC04703 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129B6-Pih1d3<tm1Hmd> |
Former Common name | Twister (flox/+) |
H-2 Haplotype | |
ES Cell line | G4 [(129S6/SvEvTac x C57BL/6NCr)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006. A PGK-neo cassette was removed by crossing with CAG-FLPe mice. |
Strain description | Pih1d3 (Stp/Twister) gene floxed mice. Expression of Pih1d3 is detected by X-gal staining. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | J. Cell Biol., 204, 203-213 (2014). 24421334 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pih1d3PIH1 domain containing 31Pih1d3<tm1Hmd>targeted mutation 1, Hiroshi HamadaSfp; sperm flagellar protein Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site1Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)1internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter lacZbeta-galactosidase (E. coli)1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter myc-tagmyc-epitope tag1myc-tag |
Ordering Information | |
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Donor DNA | phage P1 loxP, yeast FRT (flipase recombination target) site, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), E. coli lacZ, Myc-tag, mouse Pih1d3 genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Biol., 204, 203-213 (2014). |
Depositor | Hiroshi Hamada (RIKEN CDB) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |