Strain Data Sheet

RBRC04715

Strain Information

Image
BRC No.RBRC04715
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Spaca1<tm1.1Osb>/Osb
Former Common nameSpaca1 KO, B6;B6129-Spaca1<tm1.1Osb>
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2008.
Strain descriptionSpaca1 knockout mice. Spaca1 is a gene especially expressing in testis. Homozygous mutant males are infertile.
Colony maintenanceHeterozygote X Wild-type [C57BL/6NJcl]
ReferencesDevelopment, 139(19) 3583-3589 (2012). 22949614

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Spaca1sperm acrosome associated 14Spaca1<tm1.1Osb>targeted mutation 1.1, Masaru Okabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Ordering Information

Donor DNAphage P1 loxP site, mouse Spaca1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 139(19) 3583-3589 (2012).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (https://www.ccb.osaka-u.ac.jp/en/). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasaru Okabe (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data