Strain Information | |
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Image | |
BRC No. | RBRC04737 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129S2-Nmu<tm1Mko> Nms<tm1Mko> |
Former Common name | NMU/NMS-dKO |
H-2 Haplotype | |
ES Cell line | D3 [129S2/SvPas] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Masayasu Kojima, Institute of Life Science, Kurume University in 2000. D3 ES cells were used to generate the mutant mice. The mice were backcrossed to C57BL/6. |
Strain description | Nmu (Neuromedin U) and Nmus (Neuromedin S) gene double knock-out mice. Nmu KO: Exon 9 of the Nmu gene was replaced with a PGK-neo cassette. Homozygous mutant mice show a increased body weight. Nms KO: Exon 8 of the Nms gene was replaced with a neo cassette. Homozygous mutant mice show no obvious abnormality. Nmu gene knockout mice (RBRC04549), Nms gene knockout mice (RBRC04550). |
Colony maintenance | Nmu: Homozygote x Homozygote [or Crossing to C57BL/6JJcl]; Nms: Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | J. Exp. Med., 202, 217-224 (2005). 16009716 Nat. Med., 10, 1067-1073 (2004). 15448684 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Nmu genomic DNA, mouse Nms genomic DNA |
Research application | Metabolism Research |
Specific Term and Conditions | Because no paper has been published on neuromedin S knockout mice, the RECIPIENT is requested to contact the DEPOSITOR in publishing the research results obtained by use of the BIOLOGICAL RESOURCE. |
Depositor | Msayasu Kojima (Kurume University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |