Strain Data Sheet

RBRC04787

Strain Information

Image
BRC No.RBRC04787
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2(FVB)-Capn3<tm1.1Hiso>
Former Common nameB6-Capn3 KI/C129S
H-2 Haplotype
ES Cell lineE14TG2a [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Dr. Hiroyuki Sorimachi, The Tokyo Metropolitan Institute of Medical Science Rinshoken in 2007. The missense C129S mutation and a loxP flanked neomycin resistant gene were introduced into exon 3 and intron 3 of the Capn3 gene, respectively. Floxed neo was removed by crossing with FVB/N-Tg(EIIa-cre)C5379Lmgd mice. The mice were backcrossed to C57BL/6J at least 10 times.
Strain description
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesJ. Clin. Invest., 120, 2672-2683 (2010). 20592470

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Capn3calpain 32Capn3<tm1.1Hiso>targeted mutation 1.1, Hiroyuki Sorimachi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Ordering Information

Donor DNABacteriophage P1 LoxP site, Mus musculus Capn3 genomic DNA(C129S)
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. J. Clin. Invest., 120, 2672-2683 (2010).
2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
3) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research which is premised on publication.
4) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
5) In presenting the research results obtained by use of the BIOLOGICAL RESOURCE for the first time in a form of international publications, it is required that the RECIPIENT contacts the DEPOSITOR for agreement and that the DEPOSITOR and its colleagues are included as co-authors, who are designated by the DEPOSITOR.
6) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorHiroyuki Sorimachi (Tokyo Metropolitan Institute of Medical Science)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data